BioLector
The BioLector is a bench-top system for online monitoring of the growth of pro- and eucaryotic cells in a microtiter plate format (6-96 wells). For the first time users can monitor cell density, pH, pO2, temperature and expression of recombinant, fluorescent proteins in real time. Continuous and efficient shaking, even during optical measurements, allows for uninterrupted oxygen supply and unlimited growth. Operating under controlled temperature, humidity, and surface aeration the system provides cells with optimal growth conditions. Working volumes range from 100 to 1500 µl per well.
- Up to 96 mini cell cultures in parallel and with real time kinetics
- Controlled fermentations and cell cultures in MTP format
- Excellent reproducibility
- Seamless integration into liquid handling systems
Applications
- Strain and cell line selection
- Media and substrate screening
- HT protein expression
- Systems biology
- Functional genomic and proteomic studies
- Growth activity and enzyme activity tests
- Inhibition and toxicity tests
- Quality control
Technical data
| System | |
|---|---|
| Microtiter plate formats | 6, 12, 24, 48, 96 wells |
| Samples volumes | 100-1000µl (Flowerplate: 200-1500µl) |
| Ambient conditions | 15-40°C, max. 75% rH |
| Automation | Liquid handling systems |
| Power supply | 100V-240V AC (50/60 Hz), 10A/4,2A |
| Interface | Ethernet |
| Dimensions | 685x410x230 mm (WxDxH) |
| Weight | 40 kg |
| Cultivation Conditions | |
| Temperature range | 20-50°C |
| Humidity | >95% |
| Orbital shaker | 200-1800 rpm |
| kLa values | >500 1/h |
| Controlled gas atmosphere | 0-10% CO2, 0-21% O2 |
| Online Monitoring | |
| Light source | LED technology |
| Detector | Photomultiplier |
| Optical filters | 6 options |
| MTP read time | 2 min/parameter/96 wells |
| Linear biomass values | Up to 50 g/L CDW |
| Technology pO2/pH | Calibration free optodes |
| Measurement range pO2 | 0-100% air saturation |
| Measurement range pH | 5.0 - 8.5 |
| Download |  BioLector product brochure (616kb pdf-file) |
Flowerplate
The Flowerplate is a new microtiter plate which combines unique mass transfer performance with online measurements of the most relevant fermentation parameters. Based on a standard SBS footprint the flower geometry was picked out of more than 30 different well geometries. The flower shape acts in a similar way to baffles in shake flasks and dramatically increases mixing and gas/liquid mass transfer. Its performance surpasses all common microtiter plate formats providing a unique solution for cell growth and other biocatalytic reactions.
- wide volume range (0.2 – 1.5 mL)
- efficient mixing
- high mass transfer kLa >1000 1/h
- no spilling, no optical cross talk, no foaming
- multiparameter reading possible
- scalability to production fermenters
Further information
Further information on the BioLector system and the Flowerplate can be found at: www.m2p-labs.de